Application

Immunohistochemistry:
A 10-20 µg/mL concetration of a previous lot was used in IH.

Immunoprecipitation:
A previous lot of this antibody was used in IP.

Western blot:
5-20 µg/mL. Recognizes a 55 kDa band corresponding to the beta chain of the GABAA receptor.

Optimal working dilutions must be determined by end user.

Detect GABA A Receptor β 2 using this Anti-GABA A Receptor β 2, 3 Chain Antibody, clone BD17 validated for use in IH, IP & WB.

General description

Gamma-aminobutyric acid type A (GABAA) receptors are members of the neurotransmitter ligand-gated ion channels that mediate neuronal inhibition on binding GABA. The effects of GABA on GABAA receptors are modulated by a range of therapeutically important drugs, including barbiturates, anaesthetics and benzodiazepines.

Immunogen

Epitope: Extracellular domain of the beta2 & beta3 subunits of the GABAA receptor

Purified GABA/benzodiazepine receptor from bovine cortex

Legal Information

CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany

Linkage

Replaces: AB5952

Other Notes

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Physical form

Purified mouse monoclonal IgG1 liquid in buffer containing 10 mM potassium phosphate buffer pH 7.4 with 150 mM NaCl

Format: Purified

Quality

Evaluated by Western Blot on mouse brain membrane lysates.

Western Blot:
1:500 dilution of this antibody detected GABA A Receptor β 2,3 Chain on 10 µg of mouse brain membrane lysates.

Specificity

Reacts with the β-chain of the GABAA receptor.

Target description

55 kDa